INDUCTION OF HIV-1 LATENCY AND REACTIVATION IN PRIMARY MEMORY CD4 T CELLS Running Title: Ex vivo Establishment of HIV-1 Latency
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چکیده
INDUCTION OF HIV-1 LATENCY AND REACTIVATION IN PRIMARY MEMORY CD4 T CELLS Running Title: Ex vivo Establishment of HIV-1 Latency Alberto Bosque and Vicente Planelles Department of Pathology, University of Utah, Salt Lake City, UT 84132 USA Address for correspondence: Vicente Planelles, Ph.D. University of Utah School of Medicine, 15 North Medical Drive East #2100 Room 2520, Salt Lake City, Utah 84112. Phone +1-801-581-8655; Fax +1-801-587-7799; E-mail: [email protected]
منابع مشابه
Induction of HIV-1 latency and reactivation in primary memory CD4+ T cells.
The use of antiretroviral therapy in HIV type 1 (HIV-1)-infected patients does not lead to virus eradication. This is due, to a significant degree, to the fact that HIV-1 can establish a highly stable reservoir of latently infected cells. In this work, we describe an ex vivo experimental system that generates high levels of HIV-1 latently infected memory cells using primary CD4+ T cells. Using ...
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Latently infected cells form the major obstacle to HIV eradication. Studies of HIV latency have been generally hindered by the lack of a robust and rapidly deployable cell model that involves primary human CD4 T lymphocytes. Latently infected cell lines have proven useful, but it is unclear how closely these proliferating cells recapitulate the conditions of viral latency in non-dividing CD4 T ...
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HIV-1 establishes latency primarily by infecting activated CD4(+) T cells that later return to quiescence as memory cells. Latency allows HIV-1 to evade immune responses and to persist during antiretroviral therapy, which represents an important problem in clinical practice. The lack of a valid cellular model to study HIV-1 latency has hindered advances in the understanding of its biology. In t...
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The search for an HIV-1 cure has been greatly hindered by the presence of a viral reservoir that persists despite antiretroviral therapy (ART). Studies of HIV-1 latency in vivo are also complicated by the low proportion of latently infected cells in HIV-1 infected individuals. A number of models of HIV-1 latency have been developed to examine the signaling pathways and viral determinants of lat...
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HIV latency is a major obstacle to curing infection. Current strategies to eradicate HIV aim at increasing transcription of the latent provirus. In the present study we observed that latently infected CD4+ T cells from HIV-infected individuals failed to produce viral particles upon ex vivo exposure to SAHA (vorinostat), despite effective inhibition of histone deacetylases. To identify steps tha...
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تاریخ انتشار 2008